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Hericium erinaceus improves mood and sleep disorders in patients affected by overweight or obesity: Could circulating pro-BDNF and BDNF be potential biomarkers

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When needed, immunoprecipitation was performed with lysates of transfected cells by Flag-specific antibody-coated agarose beads (Sigma, cat# F2426) or GFP-specific antibody, which was coupled to protein G beads (Cytiva, cat# 17061801) at 4 C for 8 h